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| Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression |
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| Author(s): Collins AT, Habib FK, Maitland NJ, Neal DE |
| Source: JOURNAL OF CELL SCIENCE Volume: 114 Issue: 21 Pages: 3865-3872 Published: NOV 2001 |
| Times Cited: 115 References: 33 |
| Abstract: A major impediment to our understanding of the biology of stem cells is the inability to distinguish them from their differentiating progeny. We made use of the known association of stem cells with basement membranes to isolate prostate epithelial stem cells. We show that, in vivo, putative stem cells express higher levels of the alpha (2)-integrin subunit than other cells within the basal layer. Approximately 1% of basal cells examined by confocal microscopy were integrin 'bright', and these, cells can be selected directly from the tissue on the basis, of rapid adhesion to type I collagen. This selected population has a basal phenotype, as determined by expression of CK5 and CK14 and lack of expression of the differentiation-specific markers prostate specific antigen (PSA) and prostatic acid phosphatase (PAP), and has a fourfold greater ability to form colonies in vitro than the total basal population. These putative stem cells are distinguished from other basal cells by their ability to generate prostate-like glands in vivo with morphologic and immuno-histochemical evidence of prostate-specific differentiation. These properties are consistent with a stem cell origin. Furthermore, the presence of surface integrins on prostate stem cells suggests that these cells share common pathways with stem cells in other tissues. |
| Document Type: Article |
| Language: English |
| Reprint Address: Collins, AT (reprint author), Univ Newcastle Upon Tyne, Sch Med, Dept Surg, Prostate Res Grp, Newcastle Upon Tyne NE2 4HH, Tyne & Wear England |
Addresses:
1. Univ Newcastle Upon Tyne, Sch Med, Dept Surg, Prostate Res Grp, Newcastle Upon Tyne NE2 4HH, Tyne & Wear England
2. Univ Edinburgh, Western Gen Hosp, Dept Oncol, Prostate Res Grp, Edinburgh EH4 2XU, Midlothian Scotland
3. Univ York, Dept Biol, YCR Canc Res Unit, York YO1 5YN, N Yorkshire England |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND |
| Subject Category: Cell Biology |
| IDS Number: 496HZ |
| ISSN: 0021-9533 |
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