| | |  | | | | Record from Web of Science® | | | | | | |
| Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector |
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| Author(s): Cabot RA, Kuhholzer B, Chan AWS, Lai L, Park KW, Chong KY, Schatten G, Murphy CN, Abeydeera LR, Day BN, Prather RS |
| Source: ANIMAL BIOTECHNOLOGY Volume: 12 Issue: 2 Pages: 205-+ Published: 2001 |
| Times Cited: 19 References: 13 |
| Abstract: Here we report the production of transgenic pigs that express enhanced green fluorescent protein (eGFP). Porcine oocytes were matured in vitro in a serum-free, chemically defined maturation medium, subsequently infected with a replication deficient pseudotyped retrovirus, fertilized and cultured in vitro before being transferred to a recipient female. Two litters were born from these embryo transfers; one pig from each litter was identified as transgenic and both expressed eGFP. From a tool in basic research to direct applications in production agriculture, domestic livestock capable of expressing foreign genes have many scientific applications. |
| Document Type: Article |
| Language: English |
| Reprint Address: Prather, RS (reprint author), Univ Missouri, Dept Anim Sci, 162 ASRC, Columbia, MO 65211 USA |
Addresses:
1. Univ Missouri, Dept Anim Sci, Columbia, MO 65211 USA
2. Oregon Hlth Sci Univ, Oregon Reg Primate Ctr, Portland, OR 97201 USA
3. PIC, Berkeley, CA 94710 USA |
| Publisher: MARCEL DEKKER INC, 270 MADISON AVE, NEW YORK, NY 10016 USA |
| Subject Category: Agriculture, Dairy & Animal Science; Biotechnology & Applied Microbiology |
| IDS Number: 509AU |
| ISSN: 1049-5398 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |