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| Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver |
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| Author(s): Suzuki A, Zheng YW, Kaneko S, Onodera M, Fukao K, Nakauchi H, Taniguchi H |
| Source: JOURNAL OF CELL BIOLOGY Volume: 156 Issue: 1 Pages: 173-184 Published: JAN 7 2002 |
| Times Cited: 171 References: 44 |
| Abstract: Using flow cytometry and single cell-based assays, we prospectively identified hepatic stem cells with multi-lineage differentiation potential and self-renewing capability. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing pluripotent stem cells persist in the developing mouse liver and that such cells can be induced to become cells of other organs of endodermal origin under appropriate microenvironment. Manipulation of hepatic stem cells may provide new insight into therapies for diseases of the digestive system. |
| Document Type: Article |
| Language: English |
| Reprint Address: Nakauchi, H (reprint author), Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575 Japan |
Addresses:
1. Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, Tsukuba, Ibaraki 3058575 Japan
2. Univ Tsukuba, Inst Clin Med, Dept Surg, Tsukuba, Ibaraki 3058575 Japan
3. Japan Sci & Technol Corp, Tsukuba, Ibaraki 3058575 Japan
4. Univ Tokyo, Inst Med Sci, Ctr Expt Med, Lab Stem Cell Therapy, Tokyo 1088639, Japan |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: 511GM |
| ISSN: 0021-9525 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |