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| Upregulation of nox-based NAD(P)H oxidases in restenosis after carotid injury |
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| Author(s): Szocs K, Lassegue B, Sorescu D, Hilenski LL, Valppu L, Couse TL, Wilcox JN, Quinn MT, Lambeth JD, Griendling KK |
| Source: ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY Volume: 22 Issue: 1 Pages: 21-27 Published: JAN 2002 |
| Times Cited: 197 References: 32 |
| Abstract: Restenosis, a frequent complication of coronary angioplasty, is associated with increased superoxide (O-2.(-)) production. Although the molecular identity of the responsible oxidase is unclear, an NAD(P)H oxidase appears to be involved. In smooth muscle, p22phox and 2 homologues of gp91phox, nox1 and nox4, are expressed, whereas fibroblasts contain gp91phox. To begin investigating the possibility that these oxidase components might contribute to the increased O-2.(-) that accompanies neointimal formation. we measured their expression after balloon injury of the rat carotid artery. The increase in O-2.(-) production 3 to 15 days after surgery was not due to inflammatory cell infiltration but appeared to be derived from medial and neointimal smooth muscle cells and adventitial fibroblasts. Nox1 and p22phox mRNAs were increased 2.7- and 3.6-fold, respectively, at day 3 after injury and remained elevated for 15 days. gp91Phox was increased 7 to 15 days after injury, and nox4 expression was increased 2-fold, but only at day 15 after surgery. These results confirm and extend our previous in vitro data and suggest that in the vasculature, the nox-based NAD(P)H oxidases serve different functions. This dynamic regulation of oxidase components may be critical to smooth muscle phenotypic modulation in restenosis and atherosclerosis. |
| Document Type: Article |
| Language: English |
| Reprint Address: Griendling, KK (reprint author), Emory Univ, Winship Canc Inst, Div Cardiol, WMB 319, 1639 Pierce Dr, Atlanta, GA 30322 USA |
Addresses:
1. Emory Univ, Winship Canc Inst, Div Cardiol, WMB 319, Atlanta, GA 30322 USA
2. Emory Univ, Dept Med, Atlanta, GA 30322 USA
3. Emory Univ, Div Hematol Oncol, Atlanta, GA 30322 USA
4. Emory Univ, Dept Biochem, Atlanta, GA 30322 USA
5. Montana State Univ, Dept Vet Mol Biol, Bozeman, MT 59717 USA |
| Publisher: LIPPINCOTT WILLIAMS & WILKINS, 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA |
| Subject Category: Hematology; Peripheral Vascular Disease |
| IDS Number: 513GL |
| ISSN: 1079-5642 |
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