| | |  | | | | Record from Web of Science® | | | | | | |
| Engineering polydactyl zinc-finger transcription factors |
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| Author(s): Beerli RR, Barbas CF |
| Source: NATURE BIOTECHNOLOGY Volume: 20 Issue: 2 Pages: 135-141 Published: FEB 2002 |
| Times Cited: 140 References: 60 |
| Abstract: The availability of rapid and robust methods for controlling gene function is of prime importance not only for assigning functions to newly discovered genes, but also for therapeutic intervention. Traditionally, gene function has been probed by often-laborious methods that either increase the level of a gene product or decrease it. Advances now make it possible to rapidly produce zinc-finger proteins capable of recognizing virtually any 18 bp stretch of DNA-a sequence long enough to specify a unique address in any genome. The attachment of functional domains also allows the design of tailor-made transcription factors for specific genes. Recent studies demonstrate that artificial transcription factors are capable of controlling the expression of endogenous genes in their native chromosomal context with a high degree of specificity in both animals and plants. Dominant regulatory control of expression of any endogenous gene can be achieved rapidly and can be also placed under chemical control. A wide range of potential applications is now within reach. |
| Document Type: Review |
| Language: English |
| Reprint Address: Barbas, CF (reprint author), Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA |
Addresses:
1. Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
2. Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA |
| Publisher: NATURE AMERICA INC, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: 517FT |
| ISSN: 1087-0156 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |