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| Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors |
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| Author(s): Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D |
| Source: SCIENCE Volume: 295 Issue: 5556 Pages: 868-872 Published: FEB 1 2002 |
| Times Cited: 546 References: 22 |
| Abstract: Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species. |
| Document Type: Article |
| Language: English |
| Reprint Address: Baltimore, D (reprint author), CALTECH, Div Biol, Pasadena, CA 91125 USA |
Addresses:
1. CALTECH, Div Biol, Pasadena, CA 91125 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 519NU |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |