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| Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method |
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| Author(s): Livak KJ, Schmittgen TD |
| Source: METHODS Volume: 25 Issue: 4 Pages: 402-408 Published: DEC 2001 |
| Times Cited: 8,053 References: 11 |
| Abstract: The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-DeltaDeltaCr) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-DeltaDeltaCr) method. In addition, we present the derivation and applications of two variations of the 2(-DeltaDeltaCr) method that may be useful in the analysis of real-time, quantitative PCR data. (C) 2001 Elsevier science. |
| Document Type: Article |
| Language: English |
| Reprint Address: Livak, KJ (reprint author), Washington State Univ, Dept Pharmaceut Sci, Pullman, WA 99164 USA |
Addresses:
1. Washington State Univ, Dept Pharmaceut Sci, Pullman, WA 99164 USA
2. Appl Biosyst Inc, Foster City, CA 94404 USA |
| Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| Subject Category: Biochemical Research Methods; Biochemistry & Molecular Biology |
| IDS Number: 523JD |
| ISSN: 1046-2023 |
| DOI: 10.1006/meth.2001.1262 |
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