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| HOXB4-induced expansion of adult hematopoietic stem cells ex vivo |
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| Author(s): Antonchuk J, Sauvageau G, Humphries RK |
| Source: CELL Volume: 109 Issue: 1 Pages: 39-45 Published: APR 5 2002 |
| Times Cited: 284 References: 26 |
| Abstract: Hox transcription factors have emerged as important regulators of primitive hematopoietic cell proliferation and differentiation. In particular, HOXB4 appears to be a strong positive regulator of hernatopoietic stem cell (HSC) self-renewal. Here we demonstrate the potency of HOXB4 to enable high-level ex vivo HSC expansion. Cultures of nontransduced or GFP-transduced murine bone marrow cells experienced large HSC losses over 10-14 days. In sharp contrast, cultures of HOXB4-transduced cells achieved rapid, extensive, and highly polyclonal HSC expansions, resulting in over 1000-fold higher levels relative to controls and a 40-fold net HSC increase. Importantly, these HSCs retained full lympho-myeloid repopulating potential and enhanced in vivo regenerative potential, demonstrating the feasibility of achieving significant ex vivo expansion of HSCs without functional impairment. |
| Document Type: Article |
| Language: English |
| Reprint Address: Sauvageau, G (reprint author), British Columbia Canc Agcy, Terry Fox Lab, 601 W 10th Ave, Vancouver, BC V5Z 1L3 Canada |
Addresses:
1. British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3 Canada
2. Clin Res Inst Montreal, Montreal, PQ H2W 1R7 Canada
3. Univ Montreal, Maisonneuve Rosemont Hosp, Dept Med, Div Hematol, Montreal, PQ H1T 2M2 Canada
4. Univ British Columbia, Dept Med, Vancouver, BC V6T 1Z1 Canada |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE,, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 539TY |
| ISSN: 0092-8674 |
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