| | |  | | | | Record from Web of Science® | | | | | | |
| The catalytic pathway of horseradish peroxidase at high resolution |
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| Author(s): Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J |
| Source: NATURE Volume: 417 Issue: 6887 Pages: 463-468 Published: MAY 23 2002 |
| Times Cited: 216 References: 30 |
| Abstract: A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions(1-5). Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time. |
| Document Type: Article |
| Language: English |
| Reprint Address: Hajdu, J (reprint author), Univ Uppsala, Ctr Biomed, Dept Biochem, Box 576, S-75123 Uppsala, Sweden |
Addresses:
1. Univ Uppsala, Ctr Biomed, Dept Biochem, S-75123 Uppsala, Sweden
2. Univ Sussex, Sch Biol Sci, Brighton BN1 9QG, E Sussex England
3. Univ Copenhagen, Dept Chem, Prot Struct Grp, DK-2100 Copenhagen, Denmark |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 554HZ |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |