| | |  | | | | Record from Web of Science® | | | | | | |
| Combinatorial synthesis of genetic networks |
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| Author(s): Guet CC, Elowitz MB, Hsing WH, Leibler S |
| Source: SCIENCE Volume: 296 Issue: 5572 Pages: 1466-1470 Published: MAY 24 2002 |
| Times Cited: 187 References: 18 |
| Abstract: A central problem in biology is determining how genes interact as parts of functional networks. Creation and analysis of synthetic networks, composed of well-characterized genetic elements, provide a framework for theoretical modeling. Here, with the use of a combinatorial method, a library of networks with varying connectivity was generated in Escherichia coli. These networks were composed of genes encoding the transcriptional regulators Lacl, TetR, and lambda Cl, as well as the corresponding promoters. They displayed phenotypic behaviors resembling binary logical circuits, with two chemical "inputs" and a fluorescent protein "output." Within this simple system, diverse computational functions arose through changes in network connectivity. Combinatorial synthesis provides an alternative approach for studying biological networks, as well as an efficient method for producing diverse phenotypes in vivo. |
| Document Type: Article |
| Language: English |
| Reprint Address: Leibler, S (reprint author), Rockefeller Univ, Lab Living Matter, 1230 York Ave, New York, NY 10021 USA |
Addresses:
1. Rockefeller Univ, Lab Living Matter, New York, NY 10021 USA
2. Princeton Univ, Howard Hughes Med Inst, Dept Mol Biol, Princeton, NJ 08544 USA
3. Princeton Univ, Dept Phys, Princeton, NJ 08544 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 557LP |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |