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| Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles |
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| Author(s): Andreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S |
| Source: JOURNAL OF EXPERIMENTAL MEDICINE Volume: 195 Issue: 10 Pages: 1303-1316 Published: MAY 20 2002 |
| Times Cited: 131 References: 39 |
| Abstract: The hypothesis that FasL expression by tumor cells may impair the in vivo efficacy of antitumor immune responses, through a mechanism known as 'Fas tumor counterattack,' has been recently questioned, becoming the object of an intense debate based on conflicting results. Here we definitely show that FasL is indeed detectable in the cytoplasm of melanoma cells and its expression is confined to multivesicular bodies that contain melanosomes. In these structures FasL colocalizes with both melanosomal (i.e., gp100) and lysosomal (i.e., CD63) antigens. Isolated melanosomes express FasL, as detected by Western blot and cytofluorimetry, and they can exert Fas-mediated apoptosis in Jurkat cells. We additionally show that melanosome-containing multivesicular bodies degranulate extracellularly and release FasL-bearing microvesicles, that coexpress both gp100 and CD63 and retain their functional activity in triggering Fas-dependent apoptosis of lymphoid cells. Hence our data provide evidence for a novel mechanism potentially operating in Fas tumor counterattack through the secretion of subcellular particles expressing functional FasL. Such vesicles may form a sort of front line hindering lymphocytes and other immunocompetent cells from entering neoplastic lesions and exert their antitumor activity. |
| Document Type: Article |
| Language: English |
| Reprint Address: Rivoltini, L (reprint author), Ist Nazl Tumori, Unit Immunotherapy Human Tumors, Via Venezian 1, I-20133 Milan, Italy |
Addresses:
1. Ist Nazl Tumori, Unit Immunotherapy Human Tumors, I-20133 Milan, Italy
2. Ist Nazl Tumori, Unit Preclin Chemotherapy & Pharmacol, I-20133 Milan, Italy
3. Ist Nazl Tumori, Unit Immunotherapy & Gene Therapy, I-20133 Milan, Italy
4. Ist Super Sanita, Immunol Lab, I-00161 Rome, Italy
5. Univ Roma La Sapienza, Virol Sect, Dept Expt Med & Pathol, I-00161 Rome, Italy |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Immunology; Medicine, Research & Experimental |
| IDS Number: 560ZH |
| ISSN: 0022-1007 |
| DOI: 10.1084/jem.20011624 |
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