| | |  | | | | Record from Web of Science® | | | | | | |
| Use of 16S ribosomal DNA for delineation of marine bacterioplankton species |
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| Author(s): Hagstrom A, Pommier T, Rohwer F, Simu K, Stolte W, Svensson D, Zweifel UL |
| Source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY Volume: 68 Issue: 7 Pages: 3628-3633 Published: JUL 2002 |
| Times Cited: 74 References: 30 |
| Abstract: All of the marine bacterioplankton-derived 16S ribosomal DNA sequences previously deposited in GenBank were reanalyzed to determine the number of bacterial species in the oceanic surface waters. These sequences have been entered into the database since 1990. The rate of new additions reached a peak in 1999 and subsequently leveled off, suggesting that much of the marine microbial species richness has been sampled. When the GenBank sequences were dereplicated by using 97% similarity as a cutoff, 1,117 unique ribotypes were found. Of the unique sequences, 609 came from uncultured environmental clones and 508 came from cultured bacteria. We conclude that the apparent bacterioplankton species richness is relatively low. |
| Document Type: Article |
| Language: English |
| Reprint Address: Hagstrom, A (reprint author), Kalmar Univ, S-39182 Kalmar, Sweden |
Addresses:
1. Kalmar Univ, S-39182 Kalmar, Sweden
2. San Diego State Univ, Dept Biol, San Diego, CA 92182 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA |
| Subject Category: Biotechnology & Applied Microbiology; Microbiology |
| IDS Number: 569YV |
| ISSN: 0099-2240 |
| DOI: 10.1128/AEM.68.7.3628-3633.2002 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |