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| Direct x-ray observation of a single hexagonal myofilament lattice in native myofibrils of striated muscle |
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| Author(s): Iwamoto H, Nishikawa Y, Wakayama J, Fujisawa T |
| Source: BIOPHYSICAL JOURNAL Volume: 83 Issue: 2 Pages: 1074-1081 Published: AUG 2002 |
| Times Cited: 7 References: 34 |
| Abstract: A striated muscle fiber consists of thousands of myofibrils with crystalline hexagonal myofilament lattices. Because the lattices are randomly oriented, the fiber gives rise to an equatorial x-ray diffraction pattern, which is essentially a rotary-averaged "powder diffraction," carrying only information about the distance between the lattice planes. We were able to record an x-ray diffraction pattern from a single myofilament lattice, very likely originating from a single myofibril from the flight muscle of a bumblebee, by orienting the incident x-ray microbeam along the myofibrillar axis (end-on diffraction). The pattern consisted of a number of hexagonally symmetrical diffraction spots whose originating lattice planes were readily identified. This also held true for some of the weak higher order reflections. The spot-like appearance of reflections implies that the lattice order is extremely well maintained for a distance of millimeters, covering up to a thousand of similar to2.5-mum-long sarcomeres connected in series. The results open the possibility of applying the x-ray microdiffraction technique to study many other micrometer-sized assemblies of functional biomolecules in the cell. |
| Document Type: Article |
| Language: English |
| Reprint Address: Iwamoto, H (reprint author), Japan Synchrotron Radiat Res Inst, Life & Environm Div, SPring 8, 1-1-1 Kouto, Mikazuki, Hyogo 6795198 Japan |
Addresses:
1. Japan Synchrotron Radiat Res Inst, Life & Environm Div, SPring 8, Mikazuki, Hyogo 6795198 Japan |
| Publisher: BIOPHYSICAL SOCIETY, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA |
| Subject Category: Biophysics |
| IDS Number: 579VE |
| ISSN: 0006-3495 |
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