| | |  | | | | Record from Web of Science® | | | | | | |
| Stochastic gene expression in a single cell |
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| Author(s): Elowitz MB, Levine AJ, Siggia ED, Swain PS |
| Source: SCIENCE Volume: 297 Issue: 5584 Pages: 1183-1186 Published: AUG 16 2002 |
| Times Cited: 843 References: 24 |
| Abstract: Clonal populations of cells exhibit substantial phenotypic variation. Such heterogeneity can be essential for many biological processes and is conjectured to arise from stochasticity, or noise, in gene expression. We constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated. Both stochasticity inherent in the biochemical process of gene expression (intrinsic noise) and fluctuations in other cellular components (extrinsic noise) contribute substantially to overall variation. Transcription rate, regulatory dynamics, and genetic factors control the amplitude of noise. These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation. |
| Document Type: Article |
| Language: English |
| Reprint Address: Elowitz, MB (reprint author), Rockefeller Univ, Canc Biol Lab, 1230 York Ave, New York, NY 10021 USA |
Addresses:
1. Rockefeller Univ, Canc Biol Lab, New York, NY 10021 USA
2. Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 584BP |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |