| | |  | | | | Record from Web of Science® | | | | | | |
| Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes |
|
|
| Author(s): Aparicio S, Chapman J, Stupka E, Putnam N, Chia J, Dehal P, Christoffels A, Rash S, Hoon S, Smit A, Gelpke MDS, Roach J, Oh T, Ho IY, Wong M, Detter C, Verhoef F, Predki P, Tay A, Lucas S, Richardson P, Smith SF, Clark MS, Edwards YJK, Doggett N, Zharkikh A, Tavtigian SV, Pruss D, Barnstead M, Evans C, Baden H, Powell J, Glusman G, Rowen L, Hood L, Tan YH, Elgar G, Hawkins T, Venkatesh B, Rokhsar D, Brenner S |
| Source: SCIENCE Volume: 297 Issue: 5585 Pages: 1301-1310 Published: AUG 23 2002 |
| Times Cited: 720 References: 97 |
| Abstract: The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some giant genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order. |
| Document Type: Article |
| Language: English |
| Reprint Address: Aparicio, S (reprint author), Univ Cambridge, Dept Oncol, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England |
Addresses:
1. Univ Cambridge, Dept Oncol, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England
2. Inst Mol & Cell Biol, Singapore 117609, Singapore
3. US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA
4. Inst Syst Biol, Seattle, WA 98103 USA
5. MRC UK HGMP Resource Ctr, Cambridge CB10 1SB, England
6. Los Alamos Natl Lab, Los Alamos, NM 87545 USA
7. Myriad Genet Inc, Salt Lake City, UT 84108 USA
8. Celera Genom, Rockville, MD 20850 USA
9. Paradigm Therapeut Ltd, Physiol Lab, Cambridge CB2 3EG, England
10. Salk Inst Biol Studies, La Jolla, CA 92037 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 586FL |
| ISSN: 0036-8075 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |