| | |  | | | | Record from Web of Science® | | | | | | |
| Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi |
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| Author(s): Volpe TA, Kidner C, Hall IM, Teng G, Grewal SIS, Martienssen RA |
| Source: SCIENCE Volume: 297 Issue: 5588 Pages: 1833-1837 Published: SEP 13 2002 |
| Times Cited: 833 References: 72 |
| Abstract: Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well as by modified histones, and influences both gene expression and chromosome segregation. In the fission yeast Schizosaccharomyces pombe, we deleted the argonaute, dicer, and RNA-dependent RNA polymerase gene homologs, which encode part of the machinery responsible for RNA interference (RNAi). Deletion results in the aberrant accumulation of complementary transcripts from centromeric heterochromatic repeats. This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function. We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi. |
| Document Type: Article |
| Language: English |
| Reprint Address: Martienssen, RA (reprint author), Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA |
Addresses:
1. Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
2. Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 592UJ |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1074973 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |