| | |  | | | | Record from Web of Science® | | | | | | |
| Establishment and maintenance of a heterochromatin domain |
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| Author(s): Hall IM, Shankaranarayana GD, Noma KI, Ayoub N, Cohen A, Grewal SIS |
| Source: SCIENCE Volume: 297 Issue: 5590 Pages: 2232-2237 Published: SEP 27 2002 |
| Times Cited: 374 References: 37 |
| Abstract: The higher-order assembly of chromatin imposes structural organization on the genetic information of eukaryotes and is thought to be largely determined by posttranslational modi cation of histone tails. Here, we study a 20-kilobase silent domain at the mating-type region of fission yeast as a model for heterochromatin formation. We find that, although histone H3 methylated at lysine 9 ( H3 Lys(9)) directly recruits heterochromatin protein Swi6/HP1, the critical determinant for H3 Lys(9) methylation to spread in cis and to be inherited through mitosis and meiosis is Swi6 itself. We demonstrate that a centromere-homologous repeat (cenH) present at the silent mating-type region is sufficient for heterochromatin formation at an ectopic site, and that its repressive capacity is mediated by components of the RNA interference (RNAi) machinery. Moreover, cenH and the RNAi machinery cooperate to nucleate heterochromatin assembly at the endogenous mat locus but are dispensable for its subsequent inheritance. This work defines sequential requirements for the initiation and propagation of regional heterochromatic domains. |
| Document Type: Article |
| Language: English |
| Reprint Address: Grewal, SIS (reprint author), Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA |
Addresses:
1. Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
2. Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
3. Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Mol Biol, IL-91010 Jerusalem, Israel |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 597LA |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1076466 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |