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| Intercellular transfer of the cellular prion protein |
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| Author(s): Liu T, Li RL, Pan T, Liu DC, Petersen RB, Wong BS, Gambetti P, Sy MS |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 277 Issue: 49 Pages: 47671-47678 Published: DEC 6 2002 |
| Times Cited: 46 References: 68 |
| Abstract: The cellular prion protein (PrPC) is a glycosylphosphatidylinositol (GPI)-anchored protein. We investigated whether PrPC can move from one cell to another cell in a cell model. Little PrPC transfer was detected when a PrPC expressing human neuroblastoma. cell line was cultured with the human erythroleukemia cells IA lacking PrPC. Efficient transfer of PrPC was detected with the presence of phorbol 12-myristate 13-acetate, an activator of protein kinase C. Maximum PrPC transfer was observed when both donor and recipient cells were activated. Furthermore, PrPC transfer required the GPI anchor and direct cell to cell contact. However, intercellular protein transfer is not limited to PrPC, another GPI-anchored protein, CD90, also transfers from the donor cells to acceptor cells after cellular activation. Therefore, this transfer process is GPI-anchor and cellular activation dependent. These findings suggest that the intercellular transfer of GPI-anchored proteins is a regulated process, and may have implications for the pathogenesis of prion disease. |
| Document Type: Article |
| Language: English |
| Reprint Address: Sy, MS (reprint author), Case Western Reserve Univ, Sch Med, BRB, Ctr Canc Res, Rm 933,10900 Euclid Ave, Cleveland, OH 44106 USA |
Addresses:
1. Case Western Reserve Univ, Sch Med, BRB, Ctr Canc Res, Cleveland, OH 44106 USA
2. Case Western Reserve Univ, Sch Med, Inst Pathol, Div Neuropathol, Cleveland, OH 44106 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: 622TT |
| ISSN: 0021-9258 |
| DOI: 10.1074/jbc.M207458200 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |