| | |  | | | | Record from Web of Science® | | | | | | |
| Long-term multiple color imaging of live cells using quantum dot bioconjugates |
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| Author(s): Jaiswal JK, Mattoussi H, Mauro JM, Simon SM |
| Source: NATURE BIOTECHNOLOGY Volume: 21 Issue: 1 Pages: 47-51 Published: JAN 2003 |
| Times Cited: 691 References: 23 |
| Abstract: Luminescent quantum dots (QDs)-semiconductor nanocrystals-are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells. The two approaches presented are (i) endocytic uptake of QDs and (ii) selective labeling of cell surface proteins with QDs conjugated to antibodies. Live cells labeled using these approaches were used for long-term multicolor imaging. The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development. |
| Document Type: Article |
| Language: English |
| Reprint Address: Simon, SM (reprint author), Rockefeller Univ, 1230 York Ave,Box 304, New York, NY 10021 USA |
Addresses:
1. Rockefeller Univ, New York, NY 10021 USA
2. USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA
3. USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA |
| Publisher: NATURE AMERICA INC, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: 632DV |
| ISSN: 1087-0156 |
| DOI: 10.1038/nbt.767 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |