| | |  | | | | Record from Web of Science® | | | | | | |
| Systematic functional analysis of the Caenorhabditis elegans genome using RNAi |
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| Author(s): Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, Welchman DP, Zipperlen P, Ahringer J |
| Source: NATURE Volume: 421 Issue: 6920 Pages: 231-237 Published: JAN 16 2003 |
| Times Cited: 1,011 References: 40 |
| Abstract: A principal challenge currently facing biologists is how to connect the complete DNA sequence of an organism to its development and behaviour. Large-scale targeted-deletions have been successful in defining gene functions in the single-celled yeast Saccharomyces cerevisiae, but comparable analyses have yet to be performed in an animal. Here we describe the use of RNA interference to inhibit the function of similar to86% of the 19,427 predicted genes of C. elegans. We identified mutant phenotypes for 1,722 genes, about two-thirds of which were not previously associated with a phenotype. We find that genes of similar functions are clustered in distinct, multi-megabase regions of individual chromosomes; genes in these regions tend to share transcriptional profiles. Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the connections among gene sequence, chromosomal location and gene function in C. elegans. |
| Document Type: Article |
| Language: English |
| Reprint Address: Ahringer, J (reprint author), Univ Cambridge, Wellcome Trust Canc Res UK Inst, Tennis Court Rd, Cambridge CB2 1QR, England |
Addresses:
1. Univ Cambridge, Wellcome Trust Canc Res UK Inst, Cambridge CB2 1QR, England
2. Univ Cambridge, Dept Genet, Cambridge CB2 1QR, England
3. Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England
4. EMBL, European Bioinformat Inst, Cambridge CB10 1SD, England
5. Univ Salamanca, CSIC, Ctr Invest Canc, Salamanca 37007, Spain |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 635KG |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature01278 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |