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| Identification of differentially expressed genes like cofilin2 in growing collateral arteries |
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| Author(s): Boengler K, Pipp F, Broich K, Fernandez B, Schaper W, Deindl E |
| Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 300 Issue: 3 Pages: 751-756 Published: JAN 17 2003 |
| Times Cited: 11 References: 23 |
| Abstract: Arteriogenesis, the growth of pre-existing collateral arteries, can be induced in rabbits by occlusion of the femoral artery. In order to analyze the differential gene expression in arteriogenesis. cDNA of collateral arteries 24h after femoral occlusion or sham operation was subjected to suppression subtractive hybridization (SSH). We demonstrated an upregulation of the U6 snRNA binding protein Lsm5, cytochrome b, an expressed sequence tag, and the actin-depolymerizing factor cofilin2 mRNA in collateral arteries 24h after femoral ligation. For cofilin2, we also detected an increase in the protein level and a localization predominantly in smooth muscle cells of collaterals. Simultaneously with the upregulation of cofilin2 we found a downregulation of the alpha-smooth muscle actin mRNA in growing collateral arteries. In summary, our data showed an augmented expression level of genes contributing to different fundamental processes of arteriogenesis. (C) 2002 Elsevier Science (USA). All rights reserved. |
| Document Type: Article |
| Language: English |
| Reprint Address: Boengler, K (reprint author), Max Planck Inst Physiol & Clin Res, Dept Expt Cardiol, Benekestr 2, D-61231 Bad Nauheim, Germany |
Addresses:
1. Max Planck Inst Physiol & Clin Res, Dept Expt Cardiol, D-61231 Bad Nauheim, Germany |
| Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: 639BH |
| ISSN: 0006-291X |
| DOI: 10.1016/S0006-291X(02)02921-2 |
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