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| Probing the intracellular calcium sensitivity of transmitter release during synaptic facilitation |
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| Author(s): Felmy F, Neher E, Schneggenburger R |
| Source: NEURON Volume: 37 Issue: 5 Pages: 801-811 Published: MAR 6 2003 |
| Times Cited: 67 References: 53 |
| Abstract: In nerve terminals, residual Ca2+ remaining from previous activity can cause facilitation of transmitter release by a mechanism that is still under debate. Here we show that the intracellular Ca2+ sensitivity of transmitter release at the calyx of Held is largely unchanged during facilitation, which leaves an increased microdomain Ca2+ signal as a possible mechanism for facilitation. We measured the Ca2+ dependencies of facilitation, as well as of transmitter release, to estimate the required increment in microdomain Ca2+. These measurements show that linear summation of residual and microdomain Ca2+ accounts for only 30% of the observed facilitation. However, a small degree of supra-linearity in the summation of intracellular Ca2+ signals, which might be caused by saturation of cytosolic Ca2+ buffer(s), is sufficient to explain facilitation at this CNS synapse. |
| Document Type: Article |
| Language: English |
| Reprint Address: Schneggenburger, R (reprint author), Max Planck Inst Biophys Chem, Abt Membranbiophys, Fassberg 11, D-37077 Gottingen, Germany |
Addresses:
1. Max Planck Inst Biophys Chem, Abt Membranbiophys, D-37077 Gottingen, Germany
2. Max Planck Inst Biophys Chem, AG Synapt Dynam & Modulat, D-37077 Gottingen, Germany |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Neurosciences |
| IDS Number: 653TH |
| ISSN: 0896-6273 |
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