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| Real-time analysis of clathrin-mediated endocytosis during cell migration |
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| Author(s): Rappoport JZ, Simon SM |
| Source: JOURNAL OF CELL SCIENCE Volume: 116 Issue: 5 Pages: 847-855 Published: MAR 1 2003 |
| Times Cited: 66 References: 46 |
| Abstract: Simultaneous dual-color total-internal-reflection fluorescence microscopy (TIR-FM) was performed to analyze the internalization and distribution of markers for clathrin-mediated endocytosis (clathrin, dynamin1, dynamin2 and transferrin) in migrating cells. In MDCK cells, which endogenously express dynamin2, the dynamin2-EGFP fluorescence demonstrated identical spatial and temporal behavior as clathrin both prior to and during internalization. By contrast, in the same cells, the neuronal dynamin1 only localized with clathrin just prior to endocytosis. In migrating cells, each endocytic marker was polarized towards the leading edge, away from the lagging edge. These observations suggest a re-evaluation of the functional differences between dynamin1 and dynamin2, and of the role of clathrin-mediated endocytosis in cell migration. |
| Document Type: Article |
| Language: English |
| Reprint Address: Simon, SM (reprint author), Rockefeller Univ, Lab Cellular Biophys, 1230 York Ave,Box 304, New York, NY 10021 USA |
Addresses:
1. Rockefeller Univ, Lab Cellular Biophys, New York, NY 10021 USA |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND |
| Subject Category: Cell Biology |
| IDS Number: 655AR |
| ISSN: 0021-9533 |
| DOI: 10.1242/jcs.00289 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |