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| Rap1p telomere association is not required for mitotic stability of a C(3)TA(2) telomere in yeast |
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| Author(s): Alexander MK, Zakian VA |
| Source: EMBO JOURNAL Volume: 22 Issue: 7 Pages: 1688-1696 Published: APR 1 2003 |
| Times Cited: 25 References: 60 |
| Abstract: Telomeric DNA usually consists of a repetitive sequence: C(1-3)A/TG(1-3) in yeast, and C(3)TA(2)/T(2)AG(3) in vertebrates. In yeast, the sequence-specific DNA-binding protein Rap1p is thought to be essential for telomere function. In a tlc1h mutant, the templating region of the telomerase RNA gene is altered so that telomerase adds the vertebrate telomere sequence instead of the yeast sequence to the chromosome end. A tlc1h strain has short but stable telomeres and no growth defect. We show here that Rap1p and the Rap1p-associated Rif2p did not bind to a telomere that contains purely vertebrate repeats, while the TG(1-3) single-stranded DNA binding protein Cdc13p and the normally non-telomeric protein Tbf1p did bind this telomere. A chromosome with one entirely vertebrate-sequence telomere had a wild-type loss rate, and the telomere was maintained at a short but stable length. However, this telomere was unable to silence a telomere-adjacent URA3 gene, and the strain carrying this telomere had a severe defect in meiosis. We conclude that Rap1p localization to a C(3)TA(2) telomere is not required for its essential mitotic functions. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zakian, VA (reprint author), Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA |
Addresses:
1. Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA |
| Publisher: OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 666CT |
| ISSN: 0261-4189 |
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