| | |  | | | | Record from Web of Science® | | | | | | |
| Computational and experimental identification of C-elegans microRNAs |
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| Author(s): Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J |
| Source: MOLECULAR CELL Volume: 11 Issue: 5 Pages: 1253-1263 Published: MAY 2003 |
| Times Cited: 129 References: 36 |
| Abstract: MicroRNAs (miRNAs) constitute an extensive class of noncoding RNAs that are thought to regulate the expression of target genes via complementary base-pair interactions. To date, cloning has identified over 200 miRNAs; from diverse eukaryotic organisms. Despite their success, such biochemical approaches are skewed toward identifying abundant miRNAs, unlike genome-wide, sequence-based computational predictions. We developed informatic methods to predict miRNAs in the C. elegans genome using sequence conservation and structural similarity to known miRNAs and generated 214 candidates. We confirmed the expression of four new miRNAs; by Northern blotting and used a more sensitive PCR approach to verify the expression of ten additional candidates. Based on hypotheses underlying our computational methods, we estimate that the C. elegans genome may encode between 140 and 300 miRNAs and potentially many more. |
| Document Type: Article |
| Language: English |
| Reprint Address: Ruvkun, G (reprint author), Harvard Univ, Sch Med, Lipper Ctr Computat Genet, Boston, MA 02115 USA |
Addresses:
1. Harvard Univ, Sch Med, Lipper Ctr Computat Genet, Boston, MA 02115 USA
2. Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
3. Harvard Univ, Sch Med, Boston, MA 02114 USA
4. Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 683GE |
| ISSN: 1097-2765 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |