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| Production of knockout rats using ENU mutagenesis and a yeast-based screening assay |
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| Author(s): Zan YH, Haag JD, Chen KS, Shepel LA, Wigington D, Wang YR, Hu R, Lopez-Guajardo CC, Brose HL, Porter KI, Leonard RA, Hitt AA, Schommer SL, Elegbede AF, Gould MN |
| Source: NATURE BIOTECHNOLOGY Volume: 21 Issue: 6 Pages: 645-651 Published: JUN 2003 |
| Times Cited: 82 References: 20 |
| Abstract: The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F-1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes. |
| Document Type: Article |
| Language: English |
| Reprint Address: Gould, MN (reprint author), Univ Wisconsin, McArdle Lab Canc Res, Dept Oncol, 1400 Univ Ave, Madison, WI 53706 USA |
Addresses:
1. Univ Wisconsin, McArdle Lab Canc Res, Dept Oncol, Madison, WI 53706 USA |
| Publisher: NATURE PUBLISHING GROUP, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: 684RR |
| ISSN: 1087-0156 |
| DOI: 10.1038/nbt830 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |