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| Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cells |
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| Author(s): Kawasaki H, Taira K |
| Source: NATURE Volume: 423 Issue: 6942 Pages: 838-842 Published: JUN 19 2003 |
| Times Cited: 41 References: 40 |
| Abstract: MicroRNAs (miRNAs) are phylogenetically widespread small RNAs of 18-25 nucleotides in length, and are found in animals and plants. These small RNAs can regulate gene expression at a translational level through interactions with their target messenger RNAs, and they have a role in the development of Caenorhabditis elegans and plants. Although more than two hundred miRNAs have been found in mammals, their mRNA targets remain to be identified. Here, we demonstrate that the expression of Hes1, basic helix-loop-helix transcriptional repressor, is regulated by miRNA-23 (miR-23) in NT2 cells. miR-23 is almost complementary to part of the coding region, just upstream of the termination codon, of Hes1 mRNA. Reduction in the level of miR-23 by small interfering RNAs resulted in the accumulation of Hes1, and hindered the retinoic-acid-induced neuronal differentiation of NT2 cells. Thus, our results indicate that miR-23 regulates the expression of Hes1 at the post-transcriptional level, and participates in retinoic-acid-induced neuronal differentiation of NT2 cells. |
| Document Type: Article |
| Language: English |
| Reprint Address: Kawasaki, H (reprint author), Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan |
Addresses:
1. Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
2. AIST, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562 Japan |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 691BQ |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature01730 |
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