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| A conserved transcriptional enhancer regulates RAG gene expression in developing B cells |
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| Author(s): Hsu LY, Lauring J, Liang HE, Greenbaum S, Cado D, Zhuang Y, Schlissel MS |
| Source: IMMUNITY Volume: 19 Issue: 1 Pages: 105-117 Published: JUL 2003 |
| Times Cited: 67 References: 33 |
| Abstract: Although expression of the RAG1 and RAG2 genes is essential for lymphocyte development, the mechanisms responsible for the lymphoid- and developmental stage-specific regulation of these genes are poorly understood. We have identified a novel, evolutionarily conserved transcriptional enhancer in the RAG locus, called Erag, which was essential for the expression of a chromosomal reporter gene driven by either RAG promoter. Targeted deletion of Erag in the mouse germline results in a partial block in B cell development associated with deficient V(D)J recombination, whereas T cell development appears unaffected. We found that E2A transcription factors bind to Erag in vivo and can transactivate Erag-dependent reporter constructs in cotransfected cell lines. These findings lead us to conclude that RAG transcription is regulated by distinct elements in developing B and T cells and that Erag is required for optimal levels of RAG expression in early B cell precursors but not in T cells. |
| Document Type: Article |
| Language: English |
| Reprint Address: Schlissel, MS (reprint author), Univ Calif Berkeley, Dept Mol & Cell Biol, Div Immunol, Berkeley, CA 94720 USA |
Addresses:
1. Univ Calif Berkeley, Dept Mol & Cell Biol, Div Immunol, Berkeley, CA 94720 USA
2. Johns Hopkins Univ, Sch Med, Grad Program Immunol, Baltimore, MD 21205 USA
3. Duke Univ, Med Ctr, Durham, NC 27710 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Immunology |
| IDS Number: 703BP |
| ISSN: 1074-7613 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |