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| Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals |
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| Author(s): Makarchikov AF, Lakaye B, Gulyai IE, Czerniecki J, Coumans B, Wins P, Grisar T, Bettendorff L |
| Source: CELLULAR AND MOLECULAR LIFE SCIENCES Volume: 60 Issue: 7 Pages: 1477-1488 Published: JUL 2003 |
| Times Cited: 22 References: 47 |
| Abstract: In most organisms, the main form of thiamine is the coenzyme thiamine diphosphate. Thiamine triphosphate (ThTP) is also found in low amounts in most vertebrate tissues and can phosphorylate certain proteins. Here we show that ThTP exists not only in vertebrates but is present in bacteria, fungi, plants and invertebrates. Unexpectedly, we found that in Escherichia coli as well as in Arabidopsis thaliana, ThTP was synthesized only under particular circumstances such as hypoxia (E. coli) or withering (A. thaliana). In mammalian tissues, ThTP concentrations are regulated by a specific thiamine triphosphatase that we have recently characterized. This enzyme was found only in mammals. In other organisms, ThTP can be hydrolyzed by unspecific phosphohydrolases. The occurrence of ThTP from prokaryotes to mammals suggests that it may have a basic role in cell metabolism or cell signaling. A decreased content may contribute to the symptoms observed during thiamine deficiency. |
| Document Type: Article |
| Language: English |
| Reprint Address: Bettendorff, L (reprint author), Univ Liege, Ctr Cellular & Mol Neurobiol, Pl Delcour 17, B-4020 Liege, Belgium |
Addresses:
1. Univ Liege, Ctr Cellular & Mol Neurobiol, B-4020 Liege, Belgium
2. Natl Acad Sci Belarus, Inst Biochem, Enzymol Lab, Grodno 230009, Byelarus
3. Univ Bialystok, Inst Biol, PL-15950 Bialystok, Poland |
| Publisher: BIRKHAUSER VERLAG AG, VIADUKSTRASSE 40-44, PO BOX 133, CH-4010 BASEL, SWITZERLAND |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 707YL |
| ISSN: 1420-682X |
| DOI: 10.1007/s00018-003-2185-x |
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