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| In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology |
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| Author(s): Moissl C, Rudolph C, Rachel R, Koch M, Huber R |
| Source: ARCHIVES OF MICROBIOLOGY Volume: 180 Issue: 3 Pages: 211-217 Published: 2003 |
| Times Cited: 13 References: 27 |
| Abstract: Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (similar to10degreesC), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larger quantities on polyethylene nets in nature. The microbial net population, estimated to consist of about 10,000 single pearls, can be harvested once a week and the archaeal cells selectively separated by density gradient centrifugation. As in native pearls, the archaeal cell fraction obtained consisted of a single type of coccoid cells only, 0.6 mum in diameter. This novel type of euryarchaea has been tentatively named SM1 euryarchaeon. Electron microscopy and immuno-fluorescence in situ hybridization (immuno-FISH) revealed that about 100 pili-like fibers, up to 3 mum in length, emanate radially from the surface of each cell. The SM1 euryarchaeal cells exhibited a viability of about 90%. The optimal conditions for viability were temperatures between -2degreesC and 20degreesC, pH 5-9, and low salt <LF>conditions; cell viability was independent of oxygen partial pressures. The cultures stained gram-positive, the cell wall was sensitive to SDS, EDTA and Proteinase K treatment. The cells did not exhibit the typical fluorescence for methanogens and did not contain coenzyme F-420. The G+C-content was 34.5 mol%. |
| Document Type: Article |
| Language: English |
| Reprint Address: Huber, R (reprint author), Univ Regensburg, Lehrstuhl Mikrobiol & Archaeenzentrum, Univ Str 31, D-93053 Regensburg, Germany |
Addresses:
1. Univ Regensburg, Lehrstuhl Mikrobiol & Archaeenzentrum, D-93053 Regensburg, Germany |
| Publisher: SPRINGER-VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 USA |
| Subject Category: Microbiology |
| IDS Number: 715DF |
| ISSN: 0302-8933 |
| DOI: 10.1007/s00203-003-0580-1 |
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