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| Clathrin-mediated endocytosis in AP-2-depleted cells |
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| Author(s): Motley A, Bright NA, Seaman MNJ, Robinson MS |
| Source: JOURNAL OF CELL BIOLOGY Volume: 162 Issue: 5 Pages: 909-918 Published: SEP 1 2003 |
| Times Cited: 224 References: 36 |
| Abstract: We have used RNA interference to knock down the AP-2 mu2 subunit and clathrin heavy chain to undetectable levels in HeLaM cells. Clathrin-coated pits associated with the plasma membrane were still present in the AP-2-depleted cells, but they were 12-fold less abundant than in control cells. No clathrin-coated pits or vesicles could be detected in the clathrin-depleted cells, and post-Golgi membrane compartments were swollen. Receptor-mediated endocytosis of transferrin was severely inhibited in both clathrin- and AP-2-depleted cells. Endocytosis of EGF, and of an LDL receptor chimera, were also inhibited in the clathrin-depleted cells; however, both were internalized as efficiently in the AP-2-depleted cells as in control cells. These results indicate that AP-2 is not essential for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins including the transferrin receptor. Uptake of the EGF and LDL receptors may be facilitated by alternative adaptors. |
| Document Type: Article |
| Language: English |
| Reprint Address: Robinson, MS (reprint author), Univ Cambridge, CIMR, Addenbrookes Hosp, Wellcome Trust MRC Bldg,Hills Rd, Cambridge CB2 2XY, England |
Addresses:
1. Univ Cambridge, Dept Clin Biochem, CIMR, Cambridge CB2 2XY, England |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: 718KT |
| ISSN: 0021-9525 |
| DOI: 10.1083/job.200305145 |
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