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| Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress |
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| Author(s): Maathuis FJM, Filatov V, Herzyk P, Krijger GC, Axelsen KB, Chen SX, Green BJ, Li Y, Madagan KL, Sanchez-Fernandez R, Forde BG, Palmgren MG, Rea PA, Williams LE, Sanders D, Amtmann A |
| Source: PLANT JOURNAL Volume: 35 Issue: 6 Pages: 675-692 Published: SEP 2003 |
| Times Cited: 106 References: 72 |
| Abstract: Plant nutrition critically depends on the activity of membrane transporters that translocate minerals from the soil into the plant and are responsible for their intra- and intercellular distribution. Most plant membrane transporters are encoded by multigene families whose members often exhibit overlapping expression patterns and a high degree of sequence homology. Furthermore, many inorganic nutrients are transported by more than one transporter family. These considerations, coupled with a large number of so-far non-annotated putative transporter genes, hamper our progress in understanding how the activity of specific transporters is integrated into a response to fluctuating conditions. We designed an oligonucleotide microarray representing 1096 Arabidopsis transporter genes and analysed the root transporter transcriptome over a 96-h period with respect to 80 mm NaCl, K+ starvation and Ca2+ starvation. Our data show that cation stress led to changes in transcript level of many genes across most transporter gene families. Analysis of transcriptionally modulated genes across all functional groups of transporters revealed families such as V-type ATPases and aquaporins that responded to all treatments, and families - which included putative non-selective cation channels for the NaCl treatment and metal transporters for Ca2+ starvation conditions - that responded to specific ionic environments. Several gene families including primary pumps, antiporters and aquaporins were analysed in detail with respect to the mRNA levels of different isoforms during ion stress. Cluster analysis allowed identification of distinct expression profiles, and several novel putative regulatory motifs were discovered within sets of co-expressed genes. |
| Document Type: Article |
| Language: English |
| Reprint Address: Amtmann, A (reprint author), Univ Glasgow, Plant Sci Grp, IBLS, Bower Bldg, Glasgow G12 8QQ, Lanark Scotland |
Addresses:
1. Univ Glasgow, Plant Sci Grp, IBLS, Glasgow G12 8QQ, Lanark Scotland
2. Univ York, Dept Biol, CNAP, York YO10 5YW, N Yorkshire England
3. Univ Glasgow, IBLS, SHWFGF, Glasgow G12 8QQ, Lanark Scotland
4. Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants England
5. Royal Vet & Agr Univ, Dept Plant Biol, DK-1871 Frederiksberg C, Denmark
6. Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
7. Univ Lancaster, Dept Biol Sci, Lancaster LA1 4YQ, England |
| Publisher: BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND |
| Subject Category: Plant Sciences |
| IDS Number: 719TV |
| ISSN: 0960-7412 |
| DOI: 10.1046/j.1365-313X.2003.01839.x |
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