| | |  | | | | Record from Web of Science® | | | | | | |
| Functional analysis of the subunits of the chromatin assembly factor RSF |
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| Author(s): Loyola A, Huang JY, LeRoy G, Hu SR, Wang YH, Donnelly RJ, Lane WS, Lee SC, Reinberg D |
| Source: MOLECULAR AND CELLULAR BIOLOGY Volume: 23 Issue: 19 Pages: 6759-6768 Published: OCT 2003 |
| Times Cited: 33 References: 29 |
| Abstract: The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different. |
| Document Type: Article |
| Language: English |
| Reprint Address: Reinberg, D (reprint author), Univ Med & Dent New Jersey, Howard Hughes Med Inst, Div Nucleic Acids Enzymol, Dept Biochem, Piscataway, NJ 08854 USA |
Addresses:
1. Univ Med & Dent New Jersey, Howard Hughes Med Inst, Div Nucleic Acids Enzymol, Dept Biochem, Piscataway, NJ 08854 USA
2. Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
3. Natl Taiwan Univ, Inst Mol Med, Taipei 10764, Taiwan
4. Univ Med & Dent New Jersey, New Jersey Med Sch, Mol Resource Facil, Newark, NJ 07103 USA
5. Harvard Univ, Microchem & Proteom Anal Facil, Cambridge, MA 02138 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 725CN |
| ISSN: 0270-7306 |
| DOI: 10.1128/MCB.23.19.6759-6768.2003 |
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