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| The C-elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target |
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| Author(s): Lin SY, Johnson SM, Abraham M, Vella MC, Pasquinelli A, Gamberi C, Gottlieb E, Slack FJ |
| Source: DEVELOPMENTAL CELL Volume: 4 Issue: 5 Pages: 639-650 Published: MAY 2003 |
| Times Cited: 148 References: 39 |
| Abstract: Hunchback regulates the temporal identity of neuroblasts in Drosophila. Here we show that hbl-1, the C. elegans hunchback ortholog, also controls temporal patterning. Furthermore, hbl-1 is a probable target of microRNA regulation through its 3'UTR. hbl-1 loss-of-function causes the precocious expression of adult seam cell fates. This phenotype is similar to loss-of-function of lin-41, a known target of the let-7 microRNA. Like lin-41 mutations, hbl-1 loss-of-function partially suppresses a let-7 mutation. The hbl-1 3'UTR is both necessary and sufficient to downregulate a reporter gene during development, and the let-7 and lin-4 microRNAs are both required for HBL-1/GFP downregulation. Multiple elements in the hbl-1 3'UTR show complementarity to regulatory microRNAs, suggesting that microRNAs directly control hbl-1. MicroRNAs may likewise function to regulate Drusophila hunchback during temporal patterning of the nervous system. |
| Document Type: Article |
| Language: English |
| Reprint Address: Slack, FJ (reprint author), Yale Univ, Dept Mol Cellular & Dev Biol, POB 208103, New Haven, CT 06520 USA |
Addresses:
1. Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
2. Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
3. Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
4. Univ Texas, Inst Cell & Mol Biol, Austin, TX 78712 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Cell Biology; Developmental Biology |
| IDS Number: 729RF |
| ISSN: 1534-5807 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |