| | |  | | | | Record from Web of Science® | | | | | | |
| Genome-wide RNAi analysis of growth and viability in Drosophila cells |
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| Author(s): Boutros M, Kiger AA, Armknecht S, Kerr K, Hild M, Koch B, Haas SA, Paro R, Perrimon N |
| Source: SCIENCE Volume: 303 Issue: 5659 Pages: 832-835 Published: FEB 6 2004 |
| Times Cited: 292 References: 21 |
| Abstract: A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale. |
| Document Type: Article |
| Language: English |
| Reprint Address: Perrimon, N (reprint author), Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA |
Addresses:
1. Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
2. Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
3. Univ Heidelberg, Zentrum Mol Biol, D-69120 Heidelberg, Germany
4. Max Planck Inst Mol Genet, D-14195 Berlin, Germany |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 770WF |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |