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| Basal cell carcinoma and its development: Insights from radiation-induced tumors in Ptch1-deficient mice |
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| Author(s): Mancuso M, Pazzaglia S, Tanori M, Hahn H, Merola P, Rebessi S, Atkinson MJ, Di Majo V, Covelli V, Saran A |
| Source: CANCER RESEARCH Volume: 64 Issue: 3 Pages: 934-941 Published: FEB 1 2004 |
| Times Cited: 37 References: 37 |
| Abstract: Loss-of-function mutations in Patched (Ptch1) are implicated in constitutive activation of the Sonic hedgehog pathway in human basal cell carcinomas (BCCs), and inherited Ptch1 mutations underlie basal cell nevus syndrome in which a typical feature is multiple BCC occurring with greater incidence in portals of radiotherapy. Mice in which one copy of Ptch1 is inactivated show increased susceptibility to spontaneous tumor development and hypersensitivity to radiation-induced tumorigenesis, providing an ideal in vivo model to study the typical pathologies associated with basal cell nevus syndrome. We therefore examined BCC development in control and irradiated Ptch1(neo67/+) mice. We show that unirradiated mice develop putative BCC precursor lesions, i.e., basaloid hyperproliferation areas arising from both follicular and interfollicular epithelium, and that these lesions progress to nodular and infiltrative BCCs only in irradiated mice. Data of BCC incidence, multiplicity, and latency support the notion of epidermal hyperproliferations, nodular and infiltrative BCC-like tumors representing different stages of tumor development. This is additionally supported by the pattern of p53 protein expression observed in BCC subtypes and by the finding of retention of the normal remaining Ptch1 allele in all nodular, circumscribed BCCs analyzed compared with its constant loss in infiltrative BCCs. Our data suggest chronological tumor progression from basaloid hyperproliferations to nodular and then infiltrative BCC occurring in a stepwise fashion through the accumulation of sequential genetic alterations. |
| Document Type: Article |
| Language: English |
| Reprint Address: Saran, A (reprint author), ENEA CR Casaccia, Biotechnol Unit, Via Anguillarese 301, I-00060 Rome, Italy |
Addresses:
1. ENEA, Ente Nuove Tecnol Energia & Ambiente, Ctr Ric, Biotechnol Unit, Rome, Italy
2. ENEA, Ente Nuove Tecnol Energia & Ambiente, Ctr Ric, Radiat Protect Unit, Rome, Italy
3. Univ Goettingen, Inst Human Genet, Gottingen, Germany
4. GSF, Natl Res Ctr Environm & Hlth, Inst Pathol, Munich, Germany |
| Publisher: AMER ASSOC CANCER RESEARCH, 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA |
| Subject Category: Oncology |
| IDS Number: 771VF |
| ISSN: 0008-5472 |
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