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| HIV type 1 antigen-responsive CD4(+) T-lymphocytes in exposed yet HIV type 1 seronegative Ugandans |
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| Author(s): Kebba A, Kaleebu P, Serwanga J, Rowland S, Yirrell D, Downing R, Gilmour J, Imami N, Gotch F, Whitworth J |
| Source: AIDS RESEARCH AND HUMAN RETROVIRUSES Volume: 20 Issue: 1 Pages: 67-75 Published: JAN 2004 |
| Times Cited: 11 References: 37 |
| Abstract: CD4(+) T cell help is important for the functionality of CD8(+) cytotoxic T-lymphocytes (CTLs) in limiting viral replication and may contribute to mediation of apparent resistance to HIV-1 infection in exposed seronegative (ESN) individuals. Using five HIV-1 antigens in an intracellular cytokine assay, the presence of specific antigen-responsive interferon-gamma-positive (IFN-gamma(+)) CD69(+) CD4(+) T-lymphocytes was evaluated in ESNs, their seropositive partners, and unexposed seronegative controls. Ten ESNs ( five females, five uncircumcised males) were identified from 10 HIV-1 serodiscordant couples with a history of frequent unprotected sexual intercourse. All ESNs and controls were negative on two EIAs and for HIV-1 proviral DNA. The frequency of ESNs with antigen-responsive IFN-gamma(+) CD69(+) CD4(+) T-lymphocytes ranged from three to five of eight for the different HIV-1 antigens. Six of eight ESNs tested had a positive response to at least one of the five antigens. Responses were on average 3.5 times higher among seropositives compared to ESNs and absent in the five unexposed controls. A negative correlation was noted between responses in ESNs and the plasma viral load of their seropositive spouse. Clade-specific and cross-clade reactivity were noted in both ESNs and seropositive partners tested. The findings confirm that ESNs are in a state of HIV-1-specific immune activation and suggest that HIV-1-specific IFN-gamma(+) CD69(+) CD4(+) T-lymphocytes in addition to HIV-1-specific CD8(+) CTLs already described by others are potential immunological correlates of protection from persistent HIV-1 infection. |
| Document Type: Article |
| Language: English |
| Reprint Address: Gotch, F (reprint author), Univ London Imperial Coll Sci Technol & Med, Chelsea & Westminster Hosp, Dept Immunol, 369 Fulham Rd, London SW10 9NH, England |
Addresses:
1. Univ London Imperial Coll Sci Technol & Med, Chelsea & Westminster Hosp, Dept Immunol, London SW10 9NH, England
2. UVRI, Med Res Councils Programme AIDS Uganda, Entebbe, Uganda
3. Univ Edinburgh, Ctr HIV Res, Edinburgh EH9 3JN, Midlothian Scotland
4. Ctr Dis Control & Prevent, Entebbe, Uganda |
| Publisher: MARY ANN LIEBERT INC PUBL, 2 MADISON AVENUE, LARCHMONT, NY 10538 USA |
| Subject Category: Immunology; Infectious Diseases; Virology |
| IDS Number: 772VD |
| ISSN: 0889-2229 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |