| | |  | | | | Record from Web of Science® | | | | | | |
| Mice cloned from olfactory sensory neurons |
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| Author(s): Eggan E, Baldwin K, Tackett M, Osborne J, Gogos J, Chess A, Axel R, Jaenisch R |
| Source: NATURE Volume: 428 Issue: 6978 Pages: 44-49 Published: MAR 4 2004 |
| Times Cited: 4 References: 43 |
| Abstract: Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice. |
| Document Type: Article |
| Language: English |
| Reprint Address: Jaenisch, R (reprint author), MIT, Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA |
Addresses:
1. MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
2. MIT, Dept Biol, Cambridge, MA 02142 USA
3. Columbia Univ Coll Phys & Surg, Howard Hughes Med Inst, Dept Biochem & Mol Biophys, New York, NY 10032 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 780GK |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature02375 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |