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| Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst (This article was retracted see vol 311, pg 335, Jan 20 2006) |
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| Author(s): Hwang WS, Ryu YJ, Park JH, Park ES, Lee EG, Koo JM, Jeon HY, Lee BC, Kang SK, Kim SJ, Ahn C, Hwang JH, Park KY, Cibelli JB, Moon SY |
| Source: SCIENCE Volume: 303 Issue: 5664 Pages: 1669-1674 Published: MAR 12 2004 |
| Times Cited: 318 References: 28 |
| Abstract: Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells. |
| Document Type: Article |
| Language: English |
| Reprint Address: Hwang, WS (reprint author), Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea |
Addresses:
1. Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea
2. Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151742, South Korea
3. MizMedi Hosp, Med Res Ctr, Seoul, South Korea
4. Gachon Med Sch, Inchon 417840, South Korea
5. Seoul Natl Univ, Coll Med, Seoul 110744, South Korea
6. Hanyang Univ, Sch Med, Seoul 471701, South Korea
7. Sunchon Natl Univ, Coll Nat Sci, Sunchon 540742, South Korea
8. Michigan State Univ, Dept Anim Sci Physiol, E Lansing, MI 48824 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 802GG |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1094515 |
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