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| Cln3 activates G1-specific transcription via phosphorylation of the SBF transcription bound repressor Whi5 |
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| Author(s): de Bruin RAM, McDonald WH, Kalashnikova TI, Yates J, Wittenberg C |
| Source: CELL Volume: 117 Issue: 7 Pages: 887-898 Published: JUN 25 2004 |
| Times Cited: 81 References: 54 |
| Abstract: G1-specific transcriptional activation by Cln3/CDK initiates the budding yeast cell cycle. To identify targets of Cln3/CDK, we analyzed the SBF and MBF transcription factor complexes by multidimensional protein interaction technology [MudPIT). Whi5 was identified as a stably bound component of SBF but not MBF. Inactivation of Whi5 leads to premature expression of G1-specific genes and budding, whereas overexpression retards those processes. Whi5 inactivation bypasses the requirement for Cln3 both for transcriptional activation and cell cycle initiation. Whi5 associates with G1-specific promoters via SBF during early G1 phase, then dissociates coincident with transcriptional activation. Dissociation of Whi5 is promoted by Cln3 in vivo. Cln/CDK phosphorylation of Whi5 in vitro promotes its dissociation from SBF complexes. Mutation of putative CDK phosphorylation sites, at least five of which are phosphorylated in vivo, strongly reduces SBF-dependent transcription and delays cell cycle initiation. Like mammalian Rb, Whi5 is a G1-specific transcriptional repressor antagonized by CDK. |
| Document Type: Article |
| Language: English |
| Reprint Address: Wittenberg, C (reprint author), Scripps Res Inst, Dept Mol Biol, 10550 N Torrey Pines Rd, La Jolla, CA 92037 USA |
Addresses:
1. Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
2. Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 832XE |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |