| | |  | | | | Record from Web of Science® | | | | | | |
| Single cell profiling of potentiated phospho-protein networks in cancer cells |
|
|
| Author(s): Irish JM, Hovland R, Krutzik PO, Perez OD, Bruserud O, Gjertsen BT, Nolan GP |
| Source: CELL Volume: 118 Issue: 2 Pages: 217-228 Published: JUL 23 2004 |
| Times Cited: 197 References: 36 |
| Abstract: Altered growth factor responses in phospho-protein-driven signaling networks are crucial to cancer cell survival and pathology. Profiles of cancer cell signaling networks might therefore identify mechanisms by which such cells interpret environmental cues for continued growth. Using multiparameter flow cytometry, we monitored phospho-protein responses to environmental cues in acute myeloid leukemia at the single cell level. By exposing cancer cell signaling networks to potentiating inputs, rather than relying upon the basal levels of protein phosphorylation alone, we could discern unique cancer network profiles that correlated with genetics and disease outcome. Strikingly, individual cancers manifested multiple cell subsets with unique network profiles, reflecting cancer heterogeneity at the level of signaling response. The results revealed a dramatic remodeling of signaling networks in cancer cells. Thus, single cell measurements of phospho-protein responses reveal shifts in signaling potential of a phospho-protein network, allowing for categorizing of cell network phenotypes by multidimensional molecular profiles of signaling. |
| Document Type: Article |
| Language: English |
| Reprint Address: Nolan, GP (reprint author), Stanford Univ, Baxter Lab Genet Pharmacol, Dept Microbiol & Immunol, Stanford, CA 94305 USA |
Addresses:
1. Stanford Univ, Baxter Lab Genet Pharmacol, Dept Microbiol & Immunol, Stanford, CA 94305 USA
2. Univ Bergen, Ctr Med Genet & Mol Med, Haukeland Univ Hosp, Bergen, Norway
3. Univ Bergen, Ctr Med Genet & Mol Med, Proteom Unit, Bergen, Norway
4. Haukeland Univ Hosp, Haematol Sect, Dept Internal Med, N-5021 Bergen, Norway
5. Univ Bergen, Inst Med, Haematol Sect, Bergen, Norway |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 841XQ |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |