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| Transcriptional regulatory code of a eukaryotic genome |
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| Author(s): Harbison CT, Gordon DB, Lee TI, Rinaldi NJ, Macisaac KD, Danford TW, Hannett NM, Tagne JB, Reynolds DB, Yoo J, Jennings EG, Zeitlinger J, Pokholok DK, Kellis M, Rolfe PA, Takusagawa KT, Lander ES, Gifford DK, Fraenkel E, Young RA |
| Source: NATURE Volume: 431 Issue: 7004 Pages: 99-104 Published: SEP 2 2004 |
| Times Cited: 684 References: 30 |
| Abstract: DNA-binding transcriptional regulators interpret the genome's regulatory code by binding to specific sequences to induce or repress gene expression(1). Comparative genomics has recently been used to identify potential cis-regulatory sequences within the yeast genome on the basis of phylogenetic conservation(2-6), but this information alone does not reveal if or when transcriptional regulators occupy these binding sites. We have constructed an initial map of yeast's transcriptional regulatory code by identifying the sequence elements that are bound by regulators under various conditions and that are conserved among Saccharomyces species. The organization of regulatory elements in promoters and the environment-dependent use of these elements by regulators are discussed. We find that environment-specific use of regulatory elements predicts mechanistic models for the function of a large population of yeast's transcriptional regulators. |
| Document Type: Article |
| Language: English |
| Reprint Address: Young, RA (reprint author), Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA |
Addresses:
1. Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
2. MIT, Dept Biol, Cambridge, MA 02139 USA
3. MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA
4. Broad Inst, Cambridge, MA 02139 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 850VC |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature02800 |
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