| | |  | | | | Record from Web of Science® | | | | | | |
| Proteomic analysis of native metabotropic glutamate receptor 5 protein complexes reveals novel molecular constituents |
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| Author(s): Farr CD, Gafken PR, Norbeck AD, Doneanu CE, Stapels MD, Barofsky DF, Minami M, Saugstad JA |
| Source: JOURNAL OF NEUROCHEMISTRY Volume: 91 Issue: 2 Pages: 438-450 Published: OCT 2004 |
| Times Cited: 29 References: 72 |
| Abstract: We used a proteomic approach to identify novel proteins that may regulate metabotropic glutamate receptor 5 (mGluR5) responses by direct or indirect protein interactions. This approach does not rely on the heterologous expression of proteins and offers the advantage of identifying protein interactions in a native environment. The mGluR5 protein was immunoprecipitated from rat brain lysates; co-immunoprecipitating proteins were analyzed by mass spectrometry and identified peptides were matched to protein databases to determine the correlating parent proteins. This proteomic approach revealed the interaction of mGluR5 with known regulatory proteins, as well as novel proteins that reflect previously unidentified molecular constituents of the mGluR5-signaling complex. Immunoblot analysis confirmed the interaction of high confidence proteins, such as phosphofurin acidic cluster sorting protein 1, microtubule-associated protein 2a and dynamin 1, as mGluR5-interacting proteins. These studies show that a proteomic approach can be used to identify candidate interacting proteins. This approach may be particularly useful for neurobiology applications where distinct protein interactions within a signaling complex can dramatically alter the outcome of the response to neurotransmitter release, or the disruption of normal protein interactions can lead to severe neurological and psychiatric disorders. |
| Document Type: Article |
| Language: English |
| Reprint Address: Saugstad, JA (reprint author), Legacy Hlth Syst Res Ctr, Robert S Dow Neurobiol Labs, 1225 NE 2nd Ave, Portland, OR 97232 USA |
Addresses:
1. Legacy Hlth Syst Res Ctr, Robert S Dow Neurobiol Labs, Portland, OR 97232 USA
2. Fred Hutchinson Canc Res Ctr, Proteom Facil, Seattle, WA 98104 USA
3. Univ Washington, Dept Med Chem, Seattle, WA 98195 USA
4. Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA
5. Oregon State Univ, Environm Hlth Sci Ctr, Mass Spectrometry Core Facil, Corvallis, OR 97331 USA |
| Publisher: BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Neurosciences |
| IDS Number: 856UH |
| ISSN: 0022-3042 |
| DOI: 10.1111/j.1471-4159.2004.02735.x |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |