| Genome-scale models of microbial cells: Evaluating the consequences of constraints |
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| Author(s): Price ND, Reed JL, Palsson BO |
| Source: NATURE REVIEWS MICROBIOLOGY Volume: 2 Issue: 11 Pages: 886-897 Published: NOV 2004 |
| Times Cited: 223 References: 113 |
| Abstract: Microbial cells operate under governing constraints that limit their range of possible functions. With the availability of annotated genome sequences, it has become possible to reconstruct genome-scale biochemical reaction networks for microorganisms. The imposition of governing constraints on a reconstructed biochemical network leads to the definition of achievable cellular functions. In recent years, a substantial and growing toolbox of computational analysis methods has been developed to study the characteristics and capabilities of microorganisms using a constraint-based reconstruction and analysis (COBRA) approach. This approach provides a biochemically and genetically consistent framework for the generation of hypotheses and the testing of functions of microbial cells. |
| Document Type: Review |
| Language: English |
| Reprint Address: Palsson, BO (reprint author), Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA |
Addresses:
1. Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Microbiology |
| IDS Number: 866PT |
| ISSN: 1740-1526 |
| DOI: 10.1038/nrmicro1023 |