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| Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors |
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| Author(s): Schacht V, Dadras SS, Johnson LA, Jackson DG, Hong YK, Detmar M |
| Source: AMERICAN JOURNAL OF PATHOLOGY Volume: 166 Issue: 3 Pages: 913-921 Published: MAR 2005 |
| Times Cited: 156 References: 36 |
| Abstract: The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired lymphatic vascular patterning. However, research into the biological importance of podoplanin has been hampered by the lack of a generally available antibody against the human protein, and its expression in normal-tissues and in human malignancies has remained unclear. We generated a human podoplanin-Fc fusion protein and found that the commercially available mouse monoclonal antibody D2-40 specifically recognized human podoplanin, as assessed by enzyme-linked immunosorbent assay and Western blot analyses. We found that, in addition to lymphatic endothelium, podoplanin was also expressed by peritoneal mesothelial cells, osteocytes, glandular myoepithelial cells, ependymal cells, and by stromal reticular cells and follicular dendritic cells of lymphoid organs. These findings were confirmed in normal mouse tissues with anti-podoplanin antibody 8.1.1. Podoplanin was also strongly expressed by granulosa cells in normal ovarian follicles, and by ovarian dysgerminomas and granulosa cell tumors. Although podoplanin was primarily absent from normal human epidermis, its expression was strongly induced in 22 of 28 squamous cell carcinomas studied. These findings suggest a potential role of podo-planin in tumor progression, and they also identify the first commercially available antibody for the specific staining of a defined lymphatic marker in archival human tissue sections, thereby enabling more widespread studies of tumor lymphangiogenesis in human cancers. |
| Document Type: Article |
| Language: English |
| Reprint Address: Detmar, M (reprint author), Swiss Fed Inst Technol, Inst Pharmaceut Sci, Wolfgang Pauli Str 10,HCI H303, CH-8093 Zurich, Switzerland |
Addresses:
1. Swiss Fed Inst Technol, Inst Pharmaceut Sci, CH-8093 Zurich, Switzerland
2. Massachusetts Gen Hosp, Cutaneous Biol Res Ctr, Boston, MA 02114 USA
3. Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
4. Harvard Univ, Sch Med, Charlestown, MA USA
5. John Radcliffe Hosp, Weatherall Inst Mol Med, Oxford OX3 9DU, England |
| Publisher: AMER SOC INVESTIGATIVE PATHOLOGY, INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3993 USA |
| Subject Category: Pathology |
| IDS Number: 901ND |
| ISSN: 0002-9440 |
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