| | |  | | | | Record from Web of Science® | | | | | | |
| DIP-chip: Rapid and accurate determination of DNA-binding specificity |
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| Author(s): Liu X, Noll DM, Lieb JD, Clarke ND |
| Source: GENOME RESEARCH Volume: 15 Issue: 3 Pages: 421-427 Published: MAR 2005 |
| Times Cited: 39 References: 20 |
| Abstract: We have developed a new method for determining the DNA-bincling specificity of proteins. In DIP-chip (DNA immunoprecipitation with microarray detection), protein-DNA complexes are isolated from all ill Vitro mixture of purified protein and naked genomic DNA. Whole-genome DNA microarrays are Used to identify the protein-bound DNA fragments, and the sequence of file identified fragments is Used to derive binding-site descriptions. Using objective criteria for assessing the accurracy of DNA-binding motifs, and Using yeast Leu3p as a model, we demonstrate that motifs determined by DIP-chip are as effective at predicting the location of bound proteins ill vivo as are motifs determined by conventional low-throughput ill vitro methods. |
| Document Type: Article |
| Language: English |
| Reprint Address: Clarke, ND (reprint author), Johns Hopkins Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA |
Addresses:
1. Johns Hopkins Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA
2. Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
3. Univ N Carolina, Ctr Genome Sci, Chapel Hill, NC 27599 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2924 USA |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: 902CB |
| ISSN: 1088-9051 |
| DOI: 10.1101/gr.3256505 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |