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| Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium |
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| Author(s): MacFadyen JR, Haworth O, Roberston D, Hardie D, Webster MT, Morris HR, Panico M, Sutton-Smith M, Dell A, van der Geer PR, Wienke D, Buckley CD, Isacke CM |
| Source: FEBS LETTERS Volume: 579 Issue: 12 Pages: 2569-2575 Published: MAY 9 2005 |
| Times Cited: 25 References: 26 |
| Abstract: Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies; to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| Document Type: Article |
| Language: English |
| Reprint Address: Isacke, CM (reprint author), Inst Canc Res, Breakthrough Breast Canc Res Ctr, 237 Fulham Rd, London SW3 6JB, England |
Addresses:
1. Inst Canc Res, Breakthrough Breast Canc Res Ctr, London SW3 6JB, England
2. Univ Birmingham, Sch Med, MRC, Ctr Immune Regulat, Birmingham B15 2TT, W Midlands England
3. Univ London Imperial Coll Sci Technol & Med, Dept Sci Biol, London SW7 2AZ, England
4. Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: 925KI |
| ISSN: 0014-5793 |
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