| | |  | | | | Record from Web of Science® | | | | | | |
| Global gene expression profiling of circulating tumor cells |
|
|
| Author(s): Smirnov DA, Zweitzig DR, Foulk BW, Miller MC, Doyle GV, Pienta KJ, Meropol NJ, Weiner LM, Cohen SJ, Moreno JG, Connelly MC, Terstappen LWMM, O'Hara SM |
| Source: CANCER RESEARCH Volume: 65 Issue: 12 Pages: 4993-4997 Published: JUN 15 2005 |
| Times Cited: 81 References: 13 |
| Abstract: Metastases from primary tumors are responsible for most cancer deaths. It has been shown that circulating tumor cells (CTCs) can be detected in the peripheral blood of patients with a variety of metastatic cancers and that the presence of these cells is associated with poor clinical outcomes. Characterization of CTCs in metastatic cancer patients could provide additional information to augment management of the disease. Here, we describe a novel approach for the identification of molecular markers to detect and characterize CTCs in peripheral blood. Using an integrated platform to immunomagnetically isolate and immunofluorescently detect CTCs, we obtained blood containing >= 100 CTCs from one metastatic colorectal, one metastatic prostate, and one metastatic breast cancer patient. Using the RNA extracted from the CTC-enriched portion of the sample and comparing it with the RNA extracted from the corresponding CTC-depleted portion, for the first time, global gene expression profiles from CTCs were generated and a list of cancer-specific, CTC-specific genes was obtained. Subsequently, samples immunomagnetically enriched for CTCs from 74 metastatic cancer patients and 50 normal donors were used to confirm by quantitative real-time reverse transcription-PCR CTC-specific expression of selected genes and to show that gene expression profiles for CTCs may be used to distinguish normal donors from advanced cancer patients as well as to differentiate among the three different metastatic cancers. Genes such as AGR2, S100A14, S100A16, FABP1, and others were found useful for detection of CTCs in peripheral blood of advanced cancer patients. |
| Document Type: Article |
| Language: English |
| Reprint Address: O'Hara, SM (reprint author), Immunicon Corp, Res & Dev, 3401 Masons Mill Rd,Suite 100, Huntingdon Valley, PA 19006 USA |
Addresses:
1. Immunicon Corp, Res & Dev, Huntingdon Valley, PA 19006 USA
2. Temple Univ, Sch Med, Fels Inst Canc Res, Philadelphia, PA 19122 USA
3. Fox Chase Canc Ctr, Dept Med Oncol, Philadelphia, PA 19111 USA
4. Thomas Jefferson Univ Hosp, Dept Urol, Philadelphia, PA 19107 USA
5. Univ Michigan, Sch Med, Ann Arbor, MI 48109 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA |
| Subject Category: Oncology |
| IDS Number: 934UN |
| ISSN: 0008-5472 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |