| Post-transcriptional gene silencing by siRNAs and miRNAs |
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| Author(s): Filipowicz W, Jaskiewicz L, Kolb FA, Pillai RS |
| Source: CURRENT OPINION IN STRUCTURAL BIOLOGY Volume: 15 Issue: 3 Pages: 331-341 Published: JUN 2005 |
| Times Cited: 114 References: 84 |
| Abstract: Recent years have seen a rapid increase in our understanding of how double-stranded RNA (dsRNA) and 21- to 25-nucleoticle small RNAs, microRNAs (miRNAs) and small interfering RNAs (siRNAs), control gene expression in eukaryotes. This RNA-mediated regulation generally results in sequence-specific inhibition of gene expression; this can occur at levels as different as chromatin modification and silencing, translational repression and mRNA degradation. Many details of the biogenesis and function of miRNAs and siRNAs, and of the effector complexes with which they associate have been elucidated. The first structural information on protein components of the RNA interference (RNAi) and miRNA machineries is emerging, and provides some insight into the mechanism of RNA-silencing reactions. |
| Document Type: Review |
| Language: English |
| Reprint Address: Filipowicz, W (reprint author), Friedrich Miescher Inst Biomed Res, POB 2543, CH-4002 Basel, Switzerland |
Addresses:
1. Friedrich Miescher Inst Biomed Res, CH-4002 Basel, Switzerland |
| Publisher: CURRENT BIOLOGY LTD, 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 943VA |
| ISSN: 0959-440X |
| DOI: 10.1016/j.sbi.2005.05.006 |