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| Creatine supplementation normalizes mutagenesis of mitochondrial DNA as well as functional consequences |
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| Author(s): Berneburg M, Gremmel T, Kurten V, Schroeder P, Hertel I, von Mikecz A, Wild S, Chen M, Declercq L, Matsui M, Ruzicka T, Krutmann J |
| Source: JOURNAL OF INVESTIGATIVE DERMATOLOGY Volume: 125 Issue: 2 Pages: 213-220 Published: AUG 2005 |
| Times Cited: 19 References: 40 |
| Abstract: Mutations of mitochondrial (mt) DNA play a role in neurodegeneration, normal aging, premature aging of the skin (photoaging), and tumors. We and others could demonstrate that mtDNA mutations can be induced in skin cells in vitro and in normal human skin in vivo by repetitive, sublethal ultraviolet (UV)-A-irradiation. These mutations are mediated by singlet oxygen and persist in human skin as long-term biomarkers of UV exposure. Although mtDNA exclusively encodes for the respiratory chain, involvement of the energy metabolism in mtDNA mutagenesis and a protective role of the energy precursor creatine have thus far not been shown. We assessed the amount of a marker mutation of mtDNA, the so-called common deletion, by real-time PCR. Induction of the common deletion was paralleled by a measurable decrease of oxygen consumption, mitochondrial membrane potential, and ATP content, as well as an increase of matrix metalloproteinase-1. Mitochondrial mutagenesis as well as functional consequences could be normalized by increasing intracellular creatine levels. These data indicate that increase of the energy precursor creatine protects from functionally relevant, aging-associated mutations of mitochondrial DNA. |
| Document Type: Article |
| Language: English |
| Reprint Address: Krutmann, J (reprint author), Univ Dusseldorf, Inst Umweltmed Forsch, Hennekamp 50, D-40225 Dusseldorf, Germany |
Addresses:
1. Univ Dusseldorf, Inst Umweltmed Forsch, D-40225 Dusseldorf, Germany
2. Univ Tubingen, Dept Dermatol, Tubingen, Germany
3. Estee Lauder BCC, Biol Res Dept Europe, Oevel, Belgium
4. Estee Lauder Co, Dept Biol Res, Melville, NY USA
5. Univ Dusseldorf, Dept Dermatol, D-4000 Dusseldorf, Germany |
| Publisher: BLACKWELL PUBLISHING, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND |
| Subject Category: Dermatology |
| IDS Number: 945HI |
| ISSN: 0022-202X |
| DOI: 10.1111/j.0022-202X.2005.23806.x |
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